The Feline Calicivirus (FCV) qPCR test is a diagnostic tool used to detect the presence of Feline Calicivirus in cats. This virus is a common cause of upper respiratory infections in cats and can lead to symptoms such as sneezing, nasal discharge, conjunctivitis, and oral ulcers. The quantitative Polymerase Chain Reaction (qPCR) method amplifies the viral RNA present in the sample, allowing for the detection and quantification of the virus. This test is highly sensitive and specific, making it a reliable method for diagnosing FCV infections. Early detection through qPCR can aid in timely intervention and management of the disease, reducing the risk of severe outbreaks, especially in multi-cat environments such as shelters or catteries. The test requires a sample from the cat, typically obtained via swabbing the oropharyngeal or nasal passages, or collecting conjunctival swabs. The results can guide veterinarians in deciding the appropriate treatment and isolation protocols to prevent the spread of the virus. Vaccination history and clinical signs should also be considered alongside qPCR results to form a comprehensive diagnosis.